Freie Universität Berlin
Fachbereich Physik - Institut für Experimentalphysik AG Joseph
Our group (https://www.physik.fu-berlin.de/einrichtungen/ag/ag-joseph/Research/index.html) aims to understand the mechanisms and principles of lipid translocation and protein folding underlying membrane biogenesis in bacteria using a combination of biochemistry, biophysics and electron spin resonance (ESR) spectroscopy techniques. We develop and apply in situ ESR spectroscopy methods to characterize the structure and heterogeneity of these membrane protein complexes in intact cells and native membranes. As a complementary approach, the group investigates the purified complexes using ESR spectroscopy and single particle cryo-electron microscopy (cryo-EM) in vitro. Our labs are equipped with state-of-the- art spectrometers operating in the range of 4 – 94 GHz as well as modern instruments for membrane protein biochemistry. This integrated approach enables us to elucidate the structural, dynamic and mechanistic principles governing the functionality of these membrane protein complexes, which are highly sought-after targets for new drugs.
Research assistant (Praedoc) (m/f/d)
2 positions in biophysics / spectroscopy of membrane proteins
with 65%-part-time job
limited to 30.09.2027 (end of the project)
salary grade (Entgeltgruppe) 13 TV-L FU
reference code: BIF_D2024
Tasks
Our research group at Freie Universität Berlin is looking for a PhD candidate with a biophysics or spectroscopy background to investigate the molecular mechanisms involved in cross-membrane lipid translocation and protein folding. The position is initially funded for three years by the Boehringer Ingelheim Foundation with further extension possible for a successful completion of the PhD thesis.
The successful candidate will independently design and perform experiments to investigate the structural and dynamic aspects of one of the key membrane protein complexes involved in membrane biogenesis in Gram-negative bacteria. The candidate will perform purification, site-specific labeling, biochemical/functional characterization of the protein complex and investigate the structure and heterogeneity using advanced ESR spectroscopy techniques (in situ and in vitro) and cryo-EM. Our highly interdisciplinary, collaborative, and goal-oriented environment requires a creative team-oriented individual. The candidate is expected to mentor junior students and present results at national and international scientific conferences and publish in peer-reviewed journals.
Requirements
Completed a Master degree in Physics, Biophysics, Chemistry, Biochemistry, or a related field.
Desirable:
A solid background in protein expression and purification or biomolecular spectroscopy is desired. Excellent communication skills in English and ability to work independently and as part of a team.
How to apply
For further information, please contact Prof. Dr. Benesh Joseph (benesh.joseph@fu- berlin.de/ 030-838-58916).
Applications should be sent by e-mail, together with significant documents, indicating the reference code, no later than September 23rd, 2024 in PDF format (preferably as one document) to Prof. Dr. Benesh Joseph: benesh.joseph@fu-berlin.de or postal to
Freie Universität Berlin
Fachbereich Physik
Institut für Experimentalphysik AG Joseph
Herrn Prof. Dr. Benesh
Joseph Arnimallee 14
14195 Berlin (Dahlem)
With an electronic application, you acknowledge that FU Berlin saves and processes your data. FU Berlin cannot guarantee the security of your personal data if you send your application over an unencrypted connection.
Freie Universität Berlin is an equal opportunity employer.
Facts
| Published | 03.09.2024 |
|---|---|
| Category | Research assistant |
| Location | Germany, Berlin, Berlin |
| Area of responsibility | Biology, Chemistry, Natural Sciences, Physics |
| Start date (earliest) | Earliest possible |
| Duration | limited to 30.09.2027 |
| Full/Part-time | with 65%-part-time job |
| Remuneration | salary grade (Entgeltgruppe) 13 TV-L FU |
| Working language and expected level |
|
| Homepage | https://www.fu-berlin.de/ |
Requirements
| Qualification | a completed Master degree |
|---|---|
| Field of study | Natural sciences and mathematics, Biochemistry, Biology, Chemistry, Physics |
Contact
| Reference number | BIF_D2024 |
|---|---|
| Contact person | Herr Prof. Dr. Benesh Joseph |
| Contact phone number | 030-838-58916 |
Apply
| Application deadline | 23.09.2024 |
|---|---|
| Reference number | BIF_D2024 |
| By post | Freie Universität Berlin
|
| By email | benesh.joseph@fu-berlin.de |