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Tech­ni­sche Uni­ver­sität Ber­lin - Fac­ulty II - Insti­tute of Chem­istry / Bioen­er­get­ics

Rese­arch Assist­ant - 0.5 work­ing time - salary grade E13 TV-L Ber­liner Hoch­schu­len

Work­ing field:

Research on the pro­tein-sup­por­ted caroten­oid trans­port by "Holo­loge" of the Orange Caroten­oid Pro­tein (OCP)
  • Gen­er­a­tion of cDNA con­structs for pro­tein and caroten­oid pro­duc­tion in bac­teria by molecu­lar-bio­lo­gical meth­ods, site-dir­ec­ted muta­gen­esis, recom­bin­ant PCR
  • Caroten­oid and pro­tein pro­duc­tion in bac­teria, chro­ma­to­graphic puri­fic­a­tion and pro­tein ana­lyt­ics
  • Mass spec­tro­metry on caroten­oids and caroten­oid-bind­ing pro­teins
  • Site-spe­cific muta­gen­esis for the intro­duc­tion of motifs for tar­geted fluor­es­cence labeling
  • Lipo­some pre­par­a­tions and caroten­oid trans­fer exper­i­ments
  • UV/Vis absorp­tion, CD and fluor­es­cence life­time spec­tro­scopy
  • Small-angle X-ray and neut­ron scat­ter­ing exper­i­ments (SAXS, SANS)
  • Func­tional stud­ies on caroten­oid-bind­ing pro­teins
  • Writ­ing/edit­ing of sci­entific manuscripts for pub­lic­a­tion, con­fer­ence and pro­ject reports
  • PhD thesis pre­par­a­tion is pos­sible

Require­ments:

  • Suc­cess­fully com­pleted uni­versity degree (Mas­ter, Dip­lom oder Äqui­val­ent) in Bio­phys­ics, Bio­chem­istry, Bio­lo­gical Chem­istry or Chem­istry
  • Exper­i­ence in molecu­lar bio­logy, pro­tein bio­chem­istry and chro­ma­to­graphic tech­niques (FPLC, HPLC, SEC, HIC) required
  • Expert­ise in spec­tro­scopic tech­niques (UV-Vis absorp­tion spec­tro­scopy, fluor­es­cence spec­tro­scopy
Desired:
  • Exper­i­ence with mass spec­tro­met­ric ana­lysis of pro­teins and bioor­ganic molecules (ESI-MS, LC-MS), skills in bio­phys­ical chem­istry
  • abil­ity to work in a team and to cooper­ate
  • flu­ent Eng­lish (oral and writ­ten), and basic know­ledge of Rus­sian lan­guage are advant­age­ous

How to ap­ply:

Please send your writ­ten applic­a­tion with the ref­er­ence num­ber and the usual doc­u­ments to Tech­nis­che Uni­versität Ber­lin - Der Präsid­ent - Fakultät II, Institut für Chemie, FG Bioenergetik, Prof. Dr. Friedrich, Sekr. PC 14, Straße des 17. Juni 135, 10623 Berlin or by e-mail to friedrich@chem.tu-berlin.de.

To ensure equal opportunities between women and men, applications by women with the required qualifications are explicitly desired. Qualified individuals with disabilities will be favored. The TU Berlin values the diversity of its members and is committed to the goals of equal opportunities.

Please send copies only. Original documents will not be returned.