The Charité - Universitätsmedizin Berlin is a joint medical faculty, which serves both Freie Universität Berlin and Humboldt Universität zu Berlin. As one of the largest university hospitals in Europe with an important history, it plays a leading role in research, teaching and clinical care. The Charité university hospital has also made a name for itself as a modern business with certifications in the medical, clinical and management fields.
Charité Campus Virchow-Klinikum | BIH Centrum für Therapieforschung
An adverse prenatal environment can have long-term effects on the health of the child, which can manifest much later in life and lead, for example, to the development of respiratory diseases. The molecular mechanisms transmitting early exposure effects to the phenotypic scale are incompletely understood, and appropriate in vitro systems are missing. We are particularly interested in persistent epigenetic modifications and their functional translation in response to such early exposures that might help to understand the development of respiratory complications. This Ph.D. project will complement our epidemiological studies by establishing and applying in vitro models for different early adverse exposure scenarios.
The job holder will be part of a research group focusing on epigenetic modifications and health effects. In his/her project, the job holder will establish in vitro models to investigate the consequences of chemical/particulate matter exposure. He/she will establish lung organoid cultures to study the priming effects of an adverse environment during development. Beyond organoid cell culture, different molecular biology and imaging techniques will be applied, including but not limited to e.g., pyrosequencing and qPCR. Single-cell RNA sequencing studies will complement epigenetic studies.
master degree in biology, biochemistry, molecular biotechnology or related disciplines
experiences with in vitro experimental studies and human cell culture models
fluent in spoken and written English
collaborative skills, enthusiasm, willingness to above-average commitment