Charité - Universitätsmedizin Berlin - CC14 / Dept. of Radiooncology and Radiotherapy / Translational Radiooncology Laboratory
In this DFG -funded research project “Assessing clonal evolution in head and neck squamous cell carcinoma and its contribution to tumor progression and treatment” resistance we seek to decipher biological processes promoting radioresistance using clonal barcoding techniques for ultra-deep resolution of clonal dynamics ex vivo and in vivo combined with state-of-the-art genomic and transcriptomics profiling approaches.
We expect that a precise understanding of the biology of therapy-resistant tumor cell clones and their molecular characteristics will enable us to develop new treatment modalities for therapy-resistant tumors.
Charité Campus Mitte
Are you interested in applying cutting-edge techniques for cellular barcoding and molecular profiling to understand the determinants of cancer therapy resistance?
In the project, we will use (i) extensively characterized, patient-derived HNSCC in-vivo models and three-dimensional (3D) cultures; (ii) advanced lentiviral vectors for cellular barcoding using novel barcode libraries compatible with single-cell RNA sequencing and designed for high-precision clonal tracking; (iii) many years of experience with the CRISPR-Cas9 gene scissors to elucidate the causal relationships between genetics and biology of tumor cells.
The successful applicant will generate stable replica cultures of barcoded organoid models from HNSCC patients.
He/She will analyse treatment-induced changes in the clonal spectrum together with detailed characterisation of resistant subclones using bulk and single cell DNA/RNA sequencing.
The student will identify drivers of therapy resistance ex vivo and in vivo, using suitable functional analyses to validate therapeutic candidates.
Training will be offered in organoid culture techniques, lentiviral transduction, barcode sequencing and bioinformatic analysis.
Applicants must hold a master's degree in biology, biochemistry, biotechnology, or a related field;
The successful candidates are highly motivated, ambitious, self-driven and hard-working individuals with excellent grades;
Candidates should enjoy working in an international team;
An excellent written and oral command of English is essential;
Candidates are expected to have excellent presentation and writing skills;
The ideal candidate should have strong interest in cell/tumor biology and state of the art functional genomic approaches;
Experience in basic molecular biology techniques (DNA, RNA, Protein) and next generation sequencing is beneficial;
Computational knowledge to handle and analyse NGS data (R, Bioconductor) is an advantage
Applications should include a motivation letter, CV, copy of certificates, expected availability date, a complete list of publications, and 2 references
Scientific staff is given sufficient time to carry out their own scientific work in accordance with their employment relationship.
How to apply:
Bitte senden Sie sämtliche Bewerbungsunterlagen, wie z.B. Anschreiben, Lebenslauf, Zeugnisse, Urkunden usw. als PDF oder Bilddatei unter Angabe der Kennziffer an folgende Adresse:
Charité - Universitätsmedizin Berlin
Klinik für Radioonkologie und Strahlentherapie
Prof. Dr. Ingeborg Tinhofer-Keilholz
Charitéplatz 1, 10117 Berlin