The Charité - Universitätsmedizin Berlin is a joint medical faculty, which serves both Freie Universität Berlin and Humboldt Universität zu Berlin. As one of the largest university hospitals in Europe with an important history, it plays a leading role in research, teaching and clinical care. The Charité university hospital has also made a name for itself as a modern business with certifications in the medical, clinical and management fields.
Doctoral Student (m/f)
CC06, Klinik für Radiologie | Charité Campus Mitte
Experimentelle Radiologie, Collaborative Research Center (CRC) SFB 1340 "In vivo Visualization of Extracellular Matrix Pathology" (Matrix in Vision), Project B03 "Targeting Extracellular Matrix Changes Induced by an Altered Homeostasis of Phosphate Metabolism". Within this project a doctoral position is available.
The summarized hypothesis for this project is that uremic cardiomyopathy (UC) is accompanied by a pathologic increase of the glycosaminoglycan (GAG) component of the myocardial extracellular matrix (ECM) and that these changes can be detected and characterized by in vivo Magnetic Resonance Imaging (MRI) using appropriate cationic imaging probes as well as by elastography-based in vivo magnetic resonance imaging (MRI).
Experimental work with cell cultures (e.g. THP cells, fibrocytes) under varying phosphate concentrations of the medium, analysis of the produced ECM-components, incubation with various cationic imaging probes and analysis of the binding of these probes to ECM components.
In vivo research using a model for UC (adenin fed small animal). participation in and support of in vivo imaging using small animal MRI, preparation of the tissue samples (heart and other organs) for ex vivo MRI and 1H relaxometrie, and for histochemic and immunohistochemic microscopy. Furtheron processing of tissue samples for biochemical analysis, especially for characterization of ECM components.
Assistance in the investigation of binding of cationic iron oxide nanoparticles and Gadolinium compounds to GAGs using spectroscopic and relaxometric methods.
Assistance in the investigation of the viscoelastic properties of tissues using multiscale elastography.
A special focus will be on on the elucidation of the structures of GAGs and how these structures correlate with the binding of imaging probes and the disease process.
We offer collaborative supervision from basic scientists and clinical experts, embedded in one of the most dense and innovative academic regions in Europe, including the top-rated teaching hospital Charité with Europe's largest radiological department.
We are looking for highly motivated, inquisitive, enthusiastic, and result-driven candidates for the doctoral position with an excellent academic record and prior research experience. Candidates should ideally hold Master’s degrees in Biology, Medicine or related disciplines, which are relevant for successful research in the above mentioned project.